The Gadsden flag, providing a rattlesnake and the word "Don't Tread on Me

https://collinielv260.talesignal.com/posts/civil-war-flag-battles-famous-engagements-and-their-banners

The Gadsden flag, presenting a rattlesnake and the word "Don't Tread on Me," was followed via some Confederate units as a image of resistance opposed to perceived oppression.